553 Relaying disallowed

553 Relaying disallowed

Hello Zoho,
we have configured MX, SPF and DKIM for pierfrancescobattistini.it site, but when we try to send and email to the domain, we receive always this error " 553 Relaying disallowed"
why ?

we have check all with mxtoolbox and all is right compiled....

can you help please?